General description

The pBiEx™ vectors are designed for cloning and high-level expression of proteins in both bacteria and in transiently transfected Spodoptera-derived insect cells. In E. coli, target gene transcription is regulated by the tightly controlled T7lac promoter, and a ribosome binding site (rbs) directs translation initiation. In insect cells, transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. pBiEx-1 contains N-terminal His Tag and S Tag coding sequences, and a C-terminal HSV Tag coding sequence. For sequencing or PCR of recombinants in pBiEx-1, refer to the table below to choose the appropriate primers.Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

20 µg in Plastic ampoule

Warning

Toxicity: Standard Handling (A)